Ph.D. in biological sciences with specialization in plant biology; Department of Biological Sciences, Faculty of Graduate Studies and Research, University of Alberta, Canada Faculty advisor: Dr. Enrico Scarpella
09/2016
B.Sc. in biological sciences with specialization in plant biology; Department of Biological Sciences, Faculty of Science University of Alberta, Canada
04/2010
Research experience
Post-Doctoral fellow Department of Molecular, Cell, and Developmental Biology, University of California Los Angeles, United States of America Faculty advisor: Dr. Steven E. Jacobsen
09/2016–
Ph.D. student Department of Biological Sciences, University of Alberta, Canada Faculty advisor: Dr. Enrico Scarpella
09/2010–09/2016
Research assistant Department of Biological Sciences, University of Alberta, Canada Faculty advisor: Dr. Enrico Scarpella
09/2008–08/2010
Research assistant Department of Biological Sciences, University of Alberta, Canada Faculty advisor: Dr. Michael Deyholos
05/2007–08/2008
Teaching and advising experience
Volunteer assistant for MCDB 191- Epigenetics at the University of California Los Angeles
Winter Quarter 2021Winter Quarter 2020
Supervised 8 undergraduate students at the University of California Los Angeles
2017–
Teaching assistant for Biology 207- Molecular genetics and heredity at the University of Alberta
Winter semester 2016
Teaching assistant for Botany 380- Drug Plants at the University of Alberta
Winter semester 2011
Supervised 3 undergraduate students at the University of Alberta
Spring semester 2016
Research
Patents
Jacobsen S.E., Gallego-bartolomé J., Papikian A., Gardiner J. 2019. Targeted gene demethylation in plants. Publication number: US 2019/0352654 A1 Link
Refereed publications
Gardiner J.1, Ghoshal B.1, Wang M.1, Jacobsen S.E. CRISPR-CAS mediated transcriptional control and epi-mutagenesis. Invited review for Plant Physiology. 1joint first authors. Link
Krishna, A.1, Gardiner, J.1, Donner, T.J., Scarpella E. 2021. Control of vein-forming, striped gene expression by auxin signaling. BMC Biol1joint first authors. Link
Xue Y., Zhong Z., Harris C. J., Gallego-Bartolomé J., Wang M., Picard C., Cao X., Hua S., Kwok I., Feng S., Jami-Alahmadi Y., Sha J., Gardiner J., Wohlschlegel J., Jacobsen S. E.. 2021. Arabidopsis MORC proteins function in the efficient establishment of RNA directed DNA methylation. Nature Communications Link
Ghoshal B.1 and Gardiner J.1. 2021. CRISPR-dCAS9 Based Targeted Manipulation of DNA Methylation in Plants. In CRISPR-Cas Methods Volume 2. CRISPR-Cas Methods. Springer Protocols Handbooks. (2021) 1joint first authors. Link
Liu W.1, Gallego-Bartolomé J.1, Zhou Y., Zhong Z., Wang M., Wongpalee S. P., Gardiner J., Feng S., Kuo P.H., Jacobsen S.E.. 2021 Ectopic targeting of CG DNA methylation in Arabidpsis with the bacterial SssI methyltransferase. Nature Communications. 1joint first authors. Link
Gardiner J., Zhao J.M.C., Chaffin K., Jacobsen S.E.. 2020. Promoter and Terminator Optimization for DNA Methylation Targeting in Arabidopsis. Epigenomes. Link
Gallego-Bartolomé J.1, Liu W.1, Kuo P.H., Feng S., Ghoshal B., Gardiner J., Zhao J.M.C., Park S.Y., Chory J., Jacobsen S.E.. 2019. Co-targeting RNA Polymerases IV and V Promotes Efficient De Novo DNA Methylation in Arabidopsis. Cell.1joint first authors. Link
Gallego-Bartolomé J1., Gardiner J.1, Liu W.1, Papikian A.1, Ghoshal B., Kuo H.Y., Zhao J.M., Segal D.J., Jacobsen S.E. 2018. Targeted DNA demethylation of the Arabidopsis genome using the Human TET1 catalytic domain. PNAS 1joint first authors. Link
Odat O.1, Gardiner J.1, Sawchuk M.G., Verna C., Donner T.J., Scarpella E. 2014. Characterization of an allelic series in the MONOPTEROS gene of Arabidopsis. Genesis. 1joint first authors. Link
Gardiner J.1, Donner T.J.1, Scarpella E. 2011. Simultaneous activation of SHR and ATHB8 expression defines switch to preprocambial cell state in Arabidopsis leaf development. Developmental Dynamics. 1joint first authors. Link
Gardiner J.1, Sherr I.1, Scarpella E. 2010. Expression of DOF genes identifies early stages of vascular development in Arabidopsis leaves. International Journal of Developmental Biology. 1joint first authors. Link
Pre-print publications
Krishna A.1, Gardiner J.1, Donner T.J., Scarpella E.. 2020 Control of Vein-Forming, Striped Gene-Expression by Auxin Signaling. BioRxiv 1joint first authors. Link
Oral presentations at scientific meetings
Gardiner J.1 Ghoshal B., Gallego-Bartolomé J., Liu W., Papikian A., Zhao J.M., and Jacobsen S.E. Building targeted DNA methylation tools for Cassava. Plant and Animal Genome Conference XXVIII. San Diego USA. Jan 10-15 2020.
Gardiner J.1 , Ghoshal B., Gallego-Bartolomé J., Liu W., Papikian A., Zhao J.M., and Jacobsen S.E. Targeted Manipulation of DNA methylation. UCLA Molecular, Cell, and Developmental biology retreat. Santa Barbara, USA, December 6-8 2019
Gardiner J.1, Donner T.J., Scarpella E. Control of SHORT-ROOT gene activation during early leaf development. RE Peter Conference Edmonton, Canada, Mar.5-6 2015.1presenting author
Gardiner J.1, Donner T.J., Scarpella E. Control of leaf vein formation by auxin signalling. RE Peter Conference Edmonton, Canada, Feb. 27-28 2014.1presenting author
Poster presentations at scientific meetings
Gardiner J.,1 Ghoshal B., Gallego-Bartolome J., Papikian A., Liu W., Jacobsen S.E., . CRISPR-mediated DNA demethylation targeting in plants. Genome Engineering: Frontiers of CRISPR/CAS. Cold Spring Harbor Laboratories USA. October 10-13 2019 1presenting author
Gardiner J.,1 Zhao J.M., Chaffin K.,and Jacobsen S.E., Improving targeted editing of DNA Methylation through differential expression. Howard Hughes Medical Investigator meeting Chevy Chase USA. September 17-20 2019. 1presenting authorGallego-Bartolomé J.,
Gardiner J1., Liu W., Papikian A., Ghoshal B., Kuo H.Y., Zhao J.M., Segal D.J., Jacobsen S.E. Targeted DNA demethylation of the Arabidopsis genome using the Human TET1 catalytic domain. 2018. Keystone DNA and RNA Methylation. Vancouver, Canada. January 21-25 2018. 1presenting author.
Gardiner J.1, Donner T.J., Scarpella E. Control of leaf vein formation by an auxin signaling Threshold. Plant Biology. Minneapolis, USA July. 26-30 2015.1presenting author
Gardiner J.1, Donner T.J., Scarpella E. Control of leaf vein formation by auxin signalling. RE Peter Conference Edmonton, Canada, Feb. 27-28 2014.1presenting author
Gardiner J.1, Donner T.J., Scarpella E. Control of leaf vein formation by auxin signaling. ICAR 2013 Sydney, Australia, June 24-28 2013. 1presenting author
Awards and fellowships
Fellowships
Post Graduate Scholarship (Doctoral) National Science and Engineering Research Council of Canada (NSERC)
05/2012–05/2015
Queen Elizabeth II Graduate Student Award
09/2015-04/2016
Alexander Graham Bell Canadian Graduate Scholarship (Masters) National Science and Engineering Research Council of Canada (NSERC)
05/2011–05/2012
Walter H. Johns Graduate Fellowship
09/2011–09/2012
Academic, service, and teaching recognition
Department of Biological Sciences Teaching Award
04/2016-04/2017
Interdepartmental Science Students’ Society Outstanding Teaching Assistant Award
01/2016-01/2017
Alberta Graduate Student Scholarship
02/2012–02/2013
Alberta Society of Professional Biologists Scholarship
09/2011–09/2012
President’s Doctoral Prize of Distinction
09/2014–09/2015
President’s Doctoral Prize of Distinction
09/2013–09/2014
President’s Doctoral Prize of Distinction
09/2012–09/2013
Bill Samuel Graduate Student Service Award
04/2013–04/2014
Travel awards
University of Alberta Graduate Student Association’s Professional Development Award
07/2015-07/2016
J. Gordin Kaplan Graduate Student Award
05/2015–05/2016
Dr. Edwin A Cossins Graduate Scholarship in Plant Molecular Biology
03/2015–03/2016
University of Alberta Graduate Student Association’s Professional Development Award
06/2013–06/2014
University of Alberta Department of Biological Sciences Travel Award
06/2013–06/2014
Professional service
Educational outreach
The History of Hot Chocolate in 15min or Less presented at the Mildred E. Mathias Botanical Garden’s Botany of the Holidays
05/12/2019
Judge at the Edmonton Regional Science Fair
04/9/2016
How to create new plants. Presented for Ideas Day at Mckernan junior high school Edmonton, Alberta.
02/18/2015
Plants: What are they good for and how do we modify them. Presented to grade 8-9 students from the Gramina junior high school, Spruce Grove, Alberta and grade 10-11 students at The Holy Trinity high school, Edmonton, Alberta
01/20/201505/26/2014
Science Days at Leduc and Stony Plain Brightpath learning centers. various science related demonstrations given to children 5-10 years old, Stony Plain, Alberta
08/13/201407/22/201407/04/201407/03/2014
Plant Breeding and GMOs. Presented to three grade 7 classes at Archbishop Joseph MacNeil school, Edmonton, Alberta.
04/08/2014
Committee experience
Faculty of Graduate Studies and Research Councilor – University of Alberta
09/2013–05/2015
Graduate Student Representative-Plant Biology interest group at the University of Alberta
09/2012–01/2015
Web master-Biology Graduate Students Association at the University of Alberta
12/2012–09/2014
Peer review
Ad hoc reviewer for Plant physiology, and Plants
Reviewer board member for International Journal of Molecular Sciences,